Henrik Kempe

I, Henrik Kempe, have been active in the field of chemistry and chemical engineering since 1994 when I achieved a Master of Science degree in chemical engineering. After graduating, I pursued a career in the the pharmaceutical industry (Astra Zeneca, Sweden). I worked as an analytical chemist, focusing on dose uniformity and particle size distribution analyses from the Turbohaler inhalation devices. I became well experienced in various analytical techniques including robotized high-throughput analytical equipment. I developed and validated a number of analytical methods and acquired knowledge and experience in working according to Good Laboratory Practice (GLP) rules in a highly regulated environment. Subsequently, I started my graduate studies and achieved first a Licentiate of Engineering degree in Chemical Engineering (2006) and then a PhD in Analytical Chemistry (2007). My academic research has been in the fields of mathematical modeling of chromatographic processes; computational chemistry; chemometrics (statistical experimental design and multivariate data analysis); synthesis and characterization of molecularly imprinted polymers; preparation and evaluation of novel solid phases for separation, purification, and synthesis; and synthesis and characterization of nanoparticles for nanomedical applications, such as targeted drug delivery and imaging.

My academic research has resulted in two theses, eleven published peer reviewed papers in scientific journals, one manuscript to be submitted, one book chapter, two patents, one patent application, and twenty contributions at international and national scientific symposia and meetings.

I am well experienced with the following techniques: reversed phase HPLC, straight phase HPLC, gas chromatography, thin layer chromatography, size exclusion chromatography, affinity chromatography, hydrophobic interaction chromatography, protein purification, capillary electrophoresis (CE), gel electrophoresis, UV-VIS spectrophotometry, FT-IR spectrometry, radioactive assays, isothermal titration calorimetry, modelling and simulation of chromatography, development and synthesis of selective stationary phases, synthesis of polymers, suspension polymerisation, free-radical polymerizations, particle size determination by laser diffraction, particle size determination by dynamic light scattering (DLS), particle size determination with impactors/impingers (Multistage Liquid Impactor and Anderssen Cascade impactor), measurements of emitted dose and dosing uniformity, preparative particle size fractionation by sieving, optical microscopy, electron microscopy (SEM and TEM), thermo­gravimetric analysis (TGA), atomic absorption analysis, design and development of automated laboratory systems, solid-phase synthesis, design of experiments and optimization, multivariate data analysis, chemometrics, development of molecularly imprinted polymers, microwave assisted extraction, cross-reactivity studies, solid-phase extraction (SPE), stability studies of drugs, characterization of adsorption/desorption phenomena, development of drug assays, development of analytical methods and assays, development of artificial recognition elements, dry powder aerosols, medical devices for delivery of dry powder aerosols, work in accordance with GLP/GMP, synthesis and characterization of silica-, metal-, metal oxide-, and, polymer-based nanoparticles.

Stockholm University, Sweden

February 15, 2007

PhD in Analytical Chemistry

Lund University, Lund Institute of Technology, Sweden

March 22, 2006

Technical Licentiate in Chemical Engineering

Lund University, Lund Institute of Technology, Sweden

January 7, 1994

Master of Science in Chemical Engineering

Additional Courses

• Project Management (Uppsala University, 2016, 5 ECTS)
• Applied Pharmaceutical Bioinformatics (Uppsala University, 2016, 5 ECTS)
• Applied Pharmaceutical Structural Bioinformatics (Uppsala University, 2016, 5 ECTS)
• Pharmaceutical Bioinformatics (Uppsala University, 2015, 7.5 ECTS)
• Magnetic Resonance - Spectroscopy and Imaging (Lund University, 2015, 7.5 ECTS)
• Experimental Methods and Instrumentation for Synchrotron Radiation Research (Lund University, 2014, 7.5 ECTS)
• Introduction to Accelerators and Free Electron Lasers (Lund University, 2014, 7.5 ECTS)
• Introduction to Synchrotron Radiation Based Science (Lund University, 2014, 7.5 ECTS)
• Design of Experiments and Optimization (UMETRIC, 2002)
• Multivariate Data Analysis, (UMETRICS, 2002)

Papers in scientific journals

1. H. Kempe, A. Axelsson, B. Nilsson and G. Zacchi. Simulation of chromatographic processes applied to separation of proteins,Journal of Chromatography A, 846 (1999) 1-12 (DOI: 10.1016/S0021-9673(98)01079-6)
2. P. Persson, H. Kempe, G. Zacchi and B. Nilsson. A methodology for estimation of mass transfer parameters in a detailed chromatography model based on frontal experiments. Chemical Engineering Research and Design, 82 (2004) 517-526 (DOI: 10.1205/026387604323050236)
3. H. Kempe and M. Kempe. Novel Method for the Synthesis of Molecularly Imprinted Polymer Bead Libraries, Macromolecular Rapid Communication, 25 (2004) 315-320 (DOI: 10.1002/marc.200300189)
4. P. Persson, H. Kempe, G. Zacchi and B. Nilsson. Estimation of adsorption parameters in a detailed affinity chromatography model based on shallow bed experiments. Process Biochemistry, 40 (2005) 1649-1659 (DOI: 10.1016/j.procbio.2004.06.031)
5. H. Kempe, P. Persson, A. Axelsson, B. Nilsson and G. Zacchi. Determination of diffusion coefficients of proteins in stationary phases by frontal chromatography. Biotechnology and Bioengineering, 93 (2006) 656-664 (DOI: 10.1002/bit.20738)
6. H. Kempe and M. Kempe. Development and evaluation of spherical molecularly imprinted polymers. Analytical Chemistry, 78 (2006) 3659-3666 (DOI: 10.1021/ac060068i)
7. H. Kempe and M. Kempe. Influence of Salt Ions on Binding to Molecularly Imprinted Polymers. Analytical and Bioanalytical Chemistry, 396 (2010) 1599-1606 (DOI: 10.1007/s00216-009-3329-0)
8. H. Kempe and M. Kempe. QSRR analysis of β-lactam antibiotics on a penicillin G targeted MIP stationary phase. Analytical and Bioanalytical Chemistry, 398 (2010) 3087-3096 (DOI: 10.1007/s00216-010-4254-y)
9. M. Kempe, H. Kempe, et al. Magnetic nanoparticles for implant-assisted magnetic drug targeting in thrombolytic therapy. Biomaterials, 31 (2010) 9499-9510 (DOI: 10.1016/j.biomaterials.2010.07.107)
10. H. Kempe, S.A. Kates, and M. Kempe. Nanomedicine’s promising therapy: magnetic drug targeting. Expert Rev Med Devices, 8 (2011) 291-294 (DOI: 10.1586/erd.10.94)
11. H. Kempe, A. Parareda Pujolràs, and M. Kempe. Molecularly imprinted nanocarriers for sustained release of erythromycin, Pharmaceutical Research 2015, 32(2), 375-388 (DOI: 10.1007/s11095-014-1468-2)
12. H. Kempe and M. Kempe. One-pot-one-step Water-based Green Synthesis of Polymer Supraparticles Applied to Molecular Imprinting (submitted).

Books and book chapters

1. H. Kempe. Separation of Proteins by Frontal Chromatography. Licentiate Thesis, Lund University (2006)
2. H. Kempe. Advances in Separation Science: Modelling and Experimental Determination of Chromatographic Parameters by Frontal Chromatography – Molecular Imprinting: Development of Spherical Beads and Optimization of the Formulation by Chemometrics. Doctoral Thesis, Stockholm University (2007). (Link)
3. H. Kempe and M. Kempe. Molecularly Imprinted Polymers in The Power of Functional Resins in Organic Synthesis. F. Albericio and J. Tulla-Puche, Eds., Wiley-WCH., Weinheim, Germany, 2008:15-44

Patents

1. H. Kempe and M. Kempe. Molekylavtryckta polymerkulor och metoder för framställning därav (Production method for molecularly imprinted polymers). Patent: SE 526855 (2005-11-08).
2. Maria Kempe and Henrik Kempe. Bärare för inbindning och frisättning av molekyler och metod för framställning därav (Carriers for binding and release of molecules and methods for making the same). Patent: SE 537943 (2015-12-01).
3. Maria Kempe and Henrik Kempe. Diskreta belagda nanopartiklar (Discrete coated nanoparticles). Patent application SE 1230153-7.

• Cell biology (LÄKA13) – Compulsory course for 1st year medical students at Lund University, lectures on ”Enzymes” and ”Membranes” (Fall semester 2007)
• Fluid Technology, basic course – Compulsory course for 2nd year under­graduate chemical engineering students at Lund University, seminar teaching (Spring semester 2000)
• Particle Technology – Advanced course for undergraduate chemical engineering students at Lund University, seminar and laboratory teaching (Spring semesters 1998 & 1999)
• Inorganic analytical chemistry – Compulsory course for 1st year under­graduate chemical engineering students at Lund University, laboratory teaching (Spring semesters 1992 & 1993)

• Swedish: Native language
• English: Full professional proficiency
• Danish: Fluent
• Norwegian: Fluent
• Portuguese: Basic knowledge
• Spanish: Basic knowledge
• French: Basic knowledge

• Excellent proficiency in programming in C/C++, Fortran, Pascal, Assembler, MatLab/GNU Octave and R
• Excellent proficiency in the following computer softwares: Microsoft Windows, Linux, Microsoft Office/OpenOffice/LibreOffice, Modde, Simca, GraphPad Prism, CS ChemOffice, SciFinder, PhotoShop/Gimp and Blender.

• Drivers licence class B
• Completed military service at the NBC (nuclear-biological-chemical) school of the Swedish Armed Forces, obtained the rank of lieutenant (1988–1989).
• Co-organizer of the “Swedish MIP summer workshop 2007” (Lund, June 4–5 2007).
• Referee work for Analytical Chemistry, Analytica Chimica Acta, Analytical Methods, Journal of Applied Polymer Science, and Materials.